Since campylobacteriosis is the leading bacterial food-borne illness and Campylobacer is able to form biofilm, we have review/research the adhesion of bacteria as the first / important step in their survival. New control strategies are needed to block adhesion and biofilm formation on food contact surfaces in the food industry, thus we focus here on natural antimicrobial phytochemicals. Valuable phytochemicals can be obtained directly from plant materials but also from agro-food by-products and waste materials. We reviewed also the appropriate methodologies to determine anti-adhesion effects of phytochemicals, on the mechanisms of C. jejuni adhesion, and thus possible targets for reduction and control of this foodborne pathogen in food processing environments. This study gives us overview of literature and guidelines for our further research on molecular mechanism of adhesion, bacterial interaction and biofilms.
COBISS.SI-ID: 5176952
We research the modulation of Campylobacter adhesion according to the microbial interaction in food processing, human and environment surface model. We evaluate Campylobacter adhesion to abiotic (polystyrene) and biotic (Caco-2; Acanthamoeba castellanii) surfaces when grown in co-culture with Escherichia coli and/or Listeria monocytogenes. We showed that C. jejuni in co-cultures with E. coli and L. monocytogenes present significantly higher risk than C. jejuni as mono-cultures, which need to be taken into account in risk evaluation. C. jejuni adhesion is a prerequisite for their colonization, biofilm formation, and further contamination of the environment. We can highlight that C. jejuni survival under adverse conditions adhered to different surfaces, not only as individual strains, but also in co-cultures with other bacteria like E. coli and L. monocytogenes.
COBISS.SI-ID: 5171320
We investigated the anti-Campylobacter activity of pinocembrin and its mechanism of action, as well as Campylobacter responses to pinocembrin treatment at the genetic (gene expression) and phenotypic (cell fitness, motility, metabolism) levels, using C. jejuni NCTC 11168 and a multidrug efflux system repressor mutant (11168?cmeR). Pinocembrin significantly increased cell membrane permeability of Campylobacter and on genetic level altered protein production, and redox cycle and iron metabolism. Further, pinocembrin modulates the metabolic activity of C. jejuni, and that pre-treatment of C. jejuni with pinocembrin influences its virulence potential in mice. Thus, we successfully presented innovative strategy focused on cellular mechanisms as a target of the of alternative natural antimicrobials activity.
COBISS.SI-ID: 5133688