The biofilm formation triggered by uncontrolled protein adsorption, on small-diameter vascular graft implants is one of the leading cause of infections during implantation. Herein, we report of sulphated-polysaccharide based nano-coatings as protein-repellent coatings to tackle this challenge. A simple layer-by-layer strategy to functionalize surfaces of poly(caprolactone) (PCL) with antifouling multilayers manufactured from the positively charged aminocellulose and the negatively charged cellulose sulphate was employed successfully. Single bi-layer coatings from poly-ethylenemine and fucoidan were also successfully manufactured. The multilayers formed coatings of up-to 20 µg/cm2. The zwitterionic nature of the coatings has shown to repel bovine serum albumin (BSA), ?-globulin, and fibrinogen proteins, as shown by means of a quartz crystal microbalance. Given the multiple functionalities and different physiochemical properties of polysaccharide-based multilayer coatings, this system showed to be easily extended to various material surfaces, thereby broadening its potential applicability also in microfluidics, diagnostic biosensors and others.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 22727190The biofilm formation triggered by uncontrolled protein adsorption, on small-diameter vascular graft implants is one of the leading cause of infections during implantation. Herein, we report of polysac-charide based nano-coatings as protein-repellent coatings to tackle this challenge. A simple layer-by-layer strategy to functionalize surfaces of poly(caprolactone) (PCL) with antifouling multilayers man-ufactured from the positively charged aminocellulose and the negatively charged cellulose sulphate was employed successfully. The influence of net charge of the coatings as well as the hydrophilicity of the coatings on interactions with bood proteins was evaluated. The absorption kinetics of proteins on solid surfaces were looked into detail. The multilayers formed coatings of up-to 20 µg/cm2. The zwitterionic nature of the coatings has shown to repel bovine serum albumin (BSA), ?-globulin, and fibrinogen proteins, as shown by means of a quartz crystal microbalance. Given the multiple functionalities and different physiochemical properties of polysaccharide-based multilayer coatings, this system showed to be easily extended to various material surfaces, thereby broadening its potential applicability also in microfluidics, diagnostic biosensors and others.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 22416662