The research results were published as a contribution to the 12th European Congress of Chemical Engineering and 5th European Congress of Applied Biotechnology, Florence, Italy.The activity of free laccase and cellulase from extracts after different time of fungi cultivation was studied. The optimal time of P. ostreatus cultivation to obtain the highest specific activity of laccase and cellulase was determined. Extracellular enzymes produced by P. ostreatus were immobilized in the form of CLEAs and their activity was also studied. Cultivation of P. ostreatus was successfully performed on a solid medium using wheat bran as a substrate. The activities of extracellular laccase and cellulase were the highest after 8th day of P. ostratus cultivation. Immobilization of both extracellular enzymes from P. ostreatus in the form of CLEAs was done for possible application in industrial cascade bioprocesses
B.06 OtherCOBISS.SI-ID: 22686998
Poly(3-hydroxybutyrate) is a natural polymer, produced by different bacteria, with good biocompatibility and biodegradability. Cardiovascular patches, scaffolds in tissue engineering and drug carriers are some of the possible biomedical applications of poly(3-hydroxybutyrate). In the past decade, many researchers examined the different physicochemical modifications of poly(3-hydroxybutyrate) in order to improve its properties for use in the field of bone tissue engineering. Poly(3-hydroxybutyrate) composites with hydroxyapatite and bioglass are intensively tested with animal and human osteoblasts in vitro to provide information about their biocompatibility, biodegradability and osteoinductivity. Good bone regeneration was proven when poly(3-hydroxybutyrate) patches were implanted in vivo in bone tissue of cats, minipigs and rats. This review summarizes the recent reports of in vitro and in vivo studies of pure poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate) composites with the emphasis on their bioactivity and biocompatibility with bone cells.
F.02 Acquisition of new scientific knowledgeCOBISS.SI-ID: 512907832
In this research polysaccharide aerogels were prepared for their drug delivery, implants and tissue engineering applications. The results showed, that these materials are promising drug delivery systems due to their ability to fasten the release of poor water-soluble drugs and to tune the release of water soluble drugs, either as immediate or prolonged drug carriers. Additionally, implants and scaffolds could be prepared by adjusting the processing parameters in the initial production step. Therefore, the future work will be focused on the additional testing of polysaccharide aerogels for other applications, such as food, cosmetics, etc.
F.02 Acquisition of new scientific knowledgeCOBISS.SI-ID: 22588950