To study the release patterns of protein bovine serum albumin (BSA), porous poly(?-caprolactone)-chitosan scaffolds with entrapped BSA were fabricated by using supercritical CO2 (scCO2) for its potential use in tissue engineering applications. Characterization of the prepared composite biomaterials using FTIR spectra, ESEM photo analysis and DSC analysis have been carried out.
The current work concerns solid liquid extraction from red and white grapes skin and the rosehip (Rosa canina) to obtain extracts with a high phenolic content. Extracts have been acquired using conventional extraction techniques and extraction with supercritical fluids (SCFs). The effect of extraction method and experimental parameters (time, pressure, temperature and solvent mixture) mostly believed to affect the extraction process was undertaken. The quantitative parameters studied are: total phenolic compounds, proanthocyanin content, and the phenolic constituent profile. The qualitative parameter analyzed is antioxidant capacity. The results demonstrate that the contents of the major constituents significantly varied among the different types of materials.
Hybrid aerogels based on polysaccharides - silica were prepared and characterized. Tetramethylorthosilicate (TMOS) was used as inorganic precursor and various polysaccharides (alginate, pectin, xanthan and guar) were used as organic precursors. TMOS was added to polysaccharide aqueous solutions, resulting in stable wet gels. There were no additional chemicals or cross-linkers in the process. Produced wet gels were dried under supercritical conditions with CO2 in order to preserve their structure. The nitrogen adsorption results were compared to pure polysaccharide aerogels, prepared in our previous research. It is shown, that the addition of silica to pectin, xanthan, alginate and guar significantly improved their structural properties, primarily seen in the drastic increase of the surface area.