J4-1774 — Interim report
1.
Salmonella Infantis in broiler flocks in Slovenia: the prevalence of multidrug resistant strains with high genetic homogeneity and low biofilm-forming ability

In last decade, the number of Salmonella enterica subspecies enterica serovar Infantis-positive broiler flocks has been steadily increasing in Slovenia. Since multidrug resistant S. Infantis isolates are highly prevalent in the broiler meat industry and may represent a public health concern through the food chain, we aimed at investigating the antimicrobial susceptibility, genetic diversity, and biofilm-forming ability of S. Infantis from Slovenian broiler flocks. Only 8.05% of the isolates were susceptible to all of the antimicrobial agents tested, while 88.5% of the isolates were multidrug resistant. A vast majority of the isolates showed ) 90% PFGE profile similarity. The average biofilm formation for persistent strains was higher than for presumably non-persistent strains but the difference was not significant. It seems that S. Infantis persistence on broiler farms is more related to its widespread occurrence in the broiler production chain and ineffective disinfection protocols than to its ability to form biofilm.

COBISS.SI-ID: 4756602
2.
Evaluation of human milk microbiota by 16S rRNA gene next-generation sequencing (NGS) and cultivation/MALDI-TOF mass spectrometry identification

This study represent the use of 16S metagenome analysis established by one of the project partners. We analysed 31 human milk samples from self-reported, healthy Slovenian women and compared the changes in microbiota to different factors such as mother’s age and body mass index (BMI), baby’s gender, lactation period, milk collected by manual expression or brest pump and mother’s consumption of probiotics.

COBISS.SI-ID: 4326792