A new methodology based on EPR spectroscopy, EPR spectra simulation, dHEO optimization and GHOST condensation was developed with applications on membrane domain characterization and local conformations of different proteins in physiological conditions. A correlation between membrane domain properties of cancerous cells, their adhesive properties and associated metastasis was shown. As well we were the first that used new methodology to transform SDSL EPR data into local protein conformation state description and set up the fundaments for protein structure characterization based on SDSL EPR.
COBISS.SI-ID: 20690215
We were the pioneers of magnetic resonance tomography in the Earth's magnetic field and greatly contributed to general effort in search of a simple magnetic resonance imaging method. High scientific relevance is confirmed by a publication in a top-rank journal Nature
COBISS.SI-ID: 1890404
Assuming that the fast axially directed flow promotes blood clot dissolution, a new mathematical model for dissolution of non-occlusive blood clots was developed. The model enables development of diagnostic methods based on advanced MR imaging techniques useful as prognostic criteria for thrombolysis and during the process of thrombolysis. According to our knowledge we were among the firs using MR for investigation of thrombolysis. By this method some mechanisms which influence on the development of thrombolysis were clarified.
COBISS.SI-ID: 20757799
NMR method with modulated gradients, developed in our laboratories, is a low-frequency complement to high-frequency neutron scattering measurements of translational motion of particles in dynamic systems. This method enabled us to be the first to measure the spectrum of velocity correlations of molecules in porous system and grains in fluidized granular matter. New version of this technique was developed, which enables study of translational dynamics of nanoparticles, biological samples, granular matter, dynamics in water structure, diffusion of biologically active substances
COBISS.SI-ID: 1987428
New bicyclic nitroxides and spin labeled alkyl phospholipids (SL-APL) effective antitumor agents were synthetised which are favorable tools for study of mechanisms of action of APL by nondestructive EPR spectroscopy.For the first time selective and biologically active antagonists of adenosine A1 receptors were synthesized, where successful application of microwave reaction conditions was used. The influence of doxyl moiety position in alkyl chain on lipid bilayer was studied by molecular dynamics that opened a new field of evaluation of nitroxide effect on the properties of its surrounding.
COBISS.SI-ID: 1808497