For decades it was thought that the release of chemical messengers and hormones is similar in control (spontaneous) and stimulated conditions. The results of our study revealed for the first time that under spontaneous conditions the rate of hormone release can be significantly slower. This is considered to be due to kinetic restirctions of the reversible fusion pore
COBISS.SI-ID: 1748324
For about a century it was thought that gila cells exhibit distinct functional differences in comparison to neurons. However it the last decade a number of studies indicated astrocytes have funtional similarities to neurons. Our study revealed for th efirst time that regulated exocytosis of astrocytes is similar to that in neurones: i.e. exhibits similar calcium sensitivity, but the kinetics is two orders more slower in comparison to neurons.
COBISS.SI-ID: 17672153
ATP is a chemical messengers that in astrocytes mediates cell to cell communication, however the mechanism of release of ATP from astrocytes is not clear. In this study we provide compelling evidence that ATP is stored in peptidergic vesicles , their mobility is attenuated during stimulation, and that their content is reeased into extracellular space following stimulation.
COBISS.SI-ID: 23252697
The release of chemical messengers and hormones from vesicles is thought to proceed through the formation of an initially stable fusion pore, which then expands to mediate the exit of molecules stored in the vesicle lumen. The results of our study revealed for the first time that at rest the fusion pore diameter is in the subnanometer range at rest and that tranisent exocytosis is the dominant mode of exocytosis even after stimulation, during which the effective fusion pore open time and fusion pore diameter get increased
COBISS.SI-ID: 22654169
In astrocytes, which have dimensons of several tens of micrometers, it is not clear how secretory vesicles are delivered to the fusion sites at the plasma membrane. In our study below we have demonstrated for the first time that vesicle delivery to the plasma membrane requires an intact network of cytoskeletal elements. Not only actin and microtubules, but also intermediary filaments.
COBISS.SI-ID: 22326489