Paper describes sex differences in the brain from SF-1 KO mice. Analyses of calbindin and nNOS positive cells are presented. We confirmed that many sex differences in the brain are dependent on exposure to sex steroid hormones. However, some sex differences also exist in SF-1 KO mice and these differences must arise from differences in sex chromosomes. The report is important for understanding brain sexual differentiation as it is the first report about sex differences in brain gene expression developing in complete absence of steroid hormone exposure during fetal and psotnatal development.
COBISS.SI-ID: 2895738
Development of cellular assay based on the BRET method is described for monitoring interactions between the beta-arrestin 2 and receptors with seven transmembrane domains (7TM receptors). Beta-arrestin 2 mutants are described that form more stable and longer lasting complexes with the 7TM receptors. Thus yields a prolonged life-span of the receptor/arrestin complex and increased BRET signal regardless of the nature of receptor/arrestin interaction. This method is suitable for both research and compound high-throughput screening in industry.
COBISS.SI-ID: 2018426
The mechanism of recurrent airway obstruction (RAO) in horses was investigated by measuring the membrane domain structure in phagocytes isolated from bronchoalveolar lavage fluid (BAL) and from blood of healthy and RAO horses by electron paramagnetic resonance. We assume that the merging of lipid domains observed in phagocytes from BAL of healthy horses could promote cluster formation of membrane proteins or ligands, which could trigger the activation process in phagocytes of healthy horses and consequently the physiological response that probably did not happen in phagocytes of RAO horses.
COBISS.SI-ID: 2447226
The transformation of MHC isoforms in canine skeletal muscles during growth was studied. In the skeletal muscles of newborn puppies the developmental MHC isoforms prevailed. During the first 10 days after birth, the expression of MHC-emb declined, as did that of the MHC-neo during the third week. Correspondingly, the expression of MHC-IIa, and later MHC-I increased, meanwhile the expression of the MHC-IIx became prominent in the sixth week. Fastest changeover from developmental to adult MHC isoforms was noted in diaphragm and slower in postural and locomotory muscles.
COBISS.SI-ID: 2594682
The aim of the study was to determine optimal electroporation protocol for delivery of DNA into canine skeletal muscle, using reporter plasmid for GFP and comparing 5 different protocols. Highest transfection was achieved in 2 protocols: 8 pulses, 200 V/cm, 20 ms, 1Hz and combination of 1 high-voltage (600 V/cm, 100 µs) and 4 low-voltage pulses (80 V/cm, 100 ms, 1Hz). With the latter, we also achieved successful electrotransfection of plasmid, encoding IL-12 with systemic transgene production. Results of our study suggest that electrotransfection could have potential clinical work.
COBISS.SI-ID: 552571