We carried out a detailed study of the expression of the inserted gene on leaves of T1 and T2 transgenic plants. Unstable expression of the gfp gene in the following generations of transgenic plants (mosacicism) led us to study the gene expression and gene silencing. We concluded that the most likely cause of gene silencing is the multiple number of inserted copies of the gfp gene, but the cause of expression in only some cells of otherwise genetically homogenous T2 plants remained unexplained.
COBISS.SI-ID: 4049529
Grasses and clovers are of key importance in Slovenia since they cover the majority of agricultural land. We carried out genomic research in species of clover in order to assess genome, chromosome and genetic diversity or relatedness. In this study, we were the first to report on the variability of the genome size of clover and additionally characterised them by phylogeny. We found errors in the taxonomic characterisation of some accessions received from the gene bank, and therefor further studied the question in more details.
COBISS.SI-ID: 4573561
In research of the genetic variability of olives we used two marker system for molecular analysis of local and Tuscan olive varieties. We found common genetic background of Tuscan varieties within some Slovene olives ehich were probably derived from the Central Italian region. The predominant local variety ‘Istrska belica’ was introduced to Slovenia independently. The article also well presents approaches and methods of assessing genetic variability.
COBISS.SI-ID: 3882873
The group developed a diagnostic marker for identifying the lethal pathotype of Verticillium albo-atrum. This is the first pathotypical specific diagnostic marker for Verticillium in general. The marker was tested on various species of Verticillium and related fungi and on a number of isolates of V. albo-atrum, whereby we verified its specificity. A particular success of this work is the practical application of the marker, since the European Organisation of Plant Protection has recommended our diagnostic protocol.
COBISS.SI-ID: 4092537
The map locations and effects of quantitative trait loci (QTLs) were estimated for alpha-acid content in hop (Humulus lupulus L.) using genetic linkage map constructed from a double pseudo-testcross. QTL analysis indicated 4 putative QTLs on 4 linkage groups on the female map and QTLs explained 11.9 to 24.8% of the phenotypic variance. The most promising QTL to be used in marker-assisted selection is peak co-located exactly with the AFLP marker.
COBISS.SI-ID: 4092793