Ability to inhibit the pathogenic bacteria in the gut is important criteria in the selection of probiotic strains. In publication, we examined the ability of a human isolate from our collection Lb. gasseri K7 to reduce adhesion of toxigenic E. coli O8:K88 to intestinal mucosa by the use of two models: Caco-2 cultured cells and fresh intestinal mucosa tissue obtained from piglets. In the conditions simulating exclusion and competition, the K7 strain reduced the E. coli adhesion. The results indicate the possible protective role of Lb. gasseri K7 in the infections with enterotoxigenic E. coli.
COBISS.SI-ID: 1838728
Mode of action and toxicity of T-2 toxin for different organ systems are not well known. The effects of five concentrations of toxin on performance, lipid peroxidation and genotoxicity were studied. The results indicate that impaired performance, DNA fragmentation in leukocytes and elevated serum IgA levels are dose dependent. The results could not confirm the hypothesis that oxidative stress is among the mechanisms by which toxin induces DNA damage. Results allow evaluating the toxicity of different toxin concentrations and are useful for further experiments for different organs.
COBISS.SI-ID: 2001032
We described the nucA gene, whose product belongs to exonuclease/endonuclease/phosphatase Pfam family, and is thought to be involved in the removal of misincorporated uracil from DNA and its reuse. It is surprising that nucA codes for a signal peptide and, confers non-specific DNase activity but no AP endonuclease activity, which is required for DNA repair subsequent to base excision. On the other hand, nucA homologues in related organisms from the phylum Bacteroidetes also code for signal peptides. It appears that this feature is conserved and is not a peculiarity limited to P. bryantii.
COBISS.SI-ID: 2278280
No genes, with the exception of plasmid maintenance and selection genes, have been successfully expressed from plasmids in any species of the genus Prevotella until now. It is shown that nucB, a newly cloned nuclease gene from P. bryantii, can be controllably expressed from shuttle vector pRH3 in P. bryantii strain TC1-1. nucB expression is also growth-medium dependent and this regulation presumably takes place at the translational level. His-tagged NucB was purified from P. bryantii TC1-1 culture supernatant and was shown to degrade DNA as well as RNA.
COBISS.SI-ID: 2015368
Some type of dietary fiber have influence on the intestinal metabolism of minerals, nitrogen and bile acids. The aim of the study was to determine whether the addition of pectin affects only the protein metabolism in the intestine or also in the liver and skeletal muscle. The protein synthesis and translational efficiency were increased in the pectin group; while in the liver only increased translational efficiency was established. The stimulation of protein metabolism in the intestinal tissues and liver could be one of the factors that explain the decrease in muscle protein metabolism.
COBISS.SI-ID: 2328200