Fibrillation of ?-synuclein is critical in the development of Parkinson’s disease. Disordered monomeric ?-synuclein has 3 Tyr residues in the C- and 1 in the N-terminus. Interactions between the C-terminus and the central portion of the molecule may prevent or minimize aggregation/fibrillation. Therefore we replaced Tyr with Ala. Fibril formation was completely inhibited and these observations suggest that the long-range intramolecular interactions between the N- and C-termini of ?-synuclein are likely to be crucial to the fibrillation process.
COBISS.SI-ID: 3474296
The aim of the study was to develop efficient methods to quantify lipids coverin. In a first approach we determined the mass of lipids retained in oral cavity by rinsing them out. In a second one we evaluated the thickness of the lipid coating on the tongue by direct fluorescence measurement using a dye incorporated in the oil. Finally in a third approach we measured local lipid thickness using a filter paper adsorption method. All applied methods showed results in the same range. First and second approach showed very good performance in contrast to third approach.
COBISS.SI-ID: 3414136
In 150 samples of Slovenian honeys water activity and water content were determined and a statistically significant linear correlation was found. In honeydew honeys the water activity at the same water content was higher than in flower honeys. The change of physical state from crystallized to liquid has been found to influence the water activity because crystallized samples showed a higher water activity than the same honeys after liquefaction. The difference in water activity between liquefied and crystallized honeys is higher in flower honeys than in honeydew honeys.
COBISS.SI-ID: 3414392
The influence of pH and temperature on the structural organization, fluidity and permeability of the hyperthermophilic archaeon A. pernix membrane was investigated in situ by a combination of electron paramagnetic resonance and fluorescence emission spectroscopy. The fluidity changes of the membrane with pH and temperature correlate well with the ratio between the fluorescence emission intensity of the first and third bands in the vibronic spectra of pyrene, I1/I3.
COBISS.SI-ID: 3319928
In the course of a study on artificial sweeteners, new crystal structures of cyclamic acid and some cyclamates have been determined. These structural observations, together with previous physicochemical ones on solutions, show that the relationship between sweetness and structure is not an obvious one.
COBISS.SI-ID: 28619269