We applied modelling and simulation approaches to study CYP2C9 gene expression in human hepatocytes treated with well-known CYP2C9 inducers, the steroid hormone precursor dehydroepiandrosterone (DHEA) and the synthetic glucocorticoid dexamethasone (DXM). Results suggest that in addition to the potent function of GR, PXR and CAR, estrogen receptor as an additional factor, might play a role in CYP2C9 regulation by DHEA. Additionally, the balance of DHEA sulphation-desulphation processes should also be considered in any description of DHEA-induced CYP2C9 profiles.
COBISS.SI-ID: 7564372
Using gene expression profiling with a dedicated microarray, we show that xenobiotic metabolism, PPAR alpha and adipocytokine signaling, and steroid synthesis are the pathways most affected by xenobiotic TCPOBOP in normal and hyperlipidemic mice.
COBISS.SI-ID: 25838041
This manuscript presents research fields, novel findings and new tools developed in the cytochrome P450 field using the functional genomics techniques. The most widely used method is microarray technology, which has already greatly contributed to the understanding of the cytochromes P450 function and expression.
COBISS.SI-ID: 23098073
Our results provide further insight into CAR and PXR-independent effects of phenobarbital in the liver and the crosstalk between different nuclear receptor signaling pathways.
COBISS.SI-ID: 25879001
Using the Sterolgene v0 microarray we were able to detect important changes in cholesterol homeostasis and drug metabolism caused by diet, drugs and inflammation. Fundamentally, the Sterolgene series of cDNA microarrays represents an original and dedicated tool, enabling focused and cost effective studies of cholesterol homeostasis and drug metabolism.
COBISS.SI-ID: 23814361