An introductory article to our paper was writen by three eminent American scientists (J.S. Alexander, J.M. Mathis, K. Pruitt) from LSU Health Sciences Center, Shreveport, LA, 71130, U.S.A. It was pointed out that we have investigated how tumor status and patient survival is correlated with multiple markers of MSI and LOH, and how these markers can be used prognostically. A major aim of the study was to challenge the concept of the use of single or few prognostic markers in favor of multiple markers.
COBISS.SI-ID: 3688986
We described a novel approach to determine accurately the functional significance of hMLH1 mutations in vivo, based on co-expression of human MLH1 and PMS2 in yeast cells. With chromosomal integration of the genes in the yeast, expression of both human genes was controlled by the orthologous yeast promoters and unique genetic background was assured for all variants tested. Our yeast-based in vivo system may thus be used for functional characterization of variants in cancer patients found throughout the entire coding region of the gene including those with incomplete clinical data.
COBISS.SI-ID: 4304666
Erythropoietin (Epo) appears to have multiple functions outside of hematopoiesis. Epo and its receptors (EpoR) have been identified on several non-hematopoietic cells and tissues. EpoRs have been found on several types of tumors and malignant cells. However, research regarding the role of Epo and EpoR in cancer is sometimes contradictory. Our data base associated review on EpoR is offering active links to a number of different data bases and web pages.
COBISS.SI-ID: 23209945
Efforts to increase efficacy of recombinant Epo in corecting anemias associated with cancer and other diseases, by manipulating the protein's structure have met with some success, and novel Epo-like agents as well as Epo mimetic agents are in development. The demonstration that Epo can trigger signaling in some cancer cells with, potentially, adverse effects on patient health has raised warning signs in the medical community and has gained the attention of regulatory authorities.
COBISS.SI-ID: 24260313
Both heavy chain antibodies and conventional IgGs are naturally occurring in camelidae (camels and llamas). We have shown that in contrast to usual finding, aggregation can be prevented and hipervariable domains (VHHs and VHs) of both antibody types can be efficiently produced via co-amplification of VH and VHH cDNAs. Llama VHs show high sequence and structural homology with the human VH III group constituting very interesting agents in (immuno)therapeutic applications in cancer, whereas small and stable VHHs can be efficiently immobilized on a solid support of a protein microarray.
COBISS.SI-ID: 26129369