MD-2 is a soluble protein involved in endotoxin (LPS) signalling via the TLR4 receptor. In this paper we showed that taxanes (paclitaxela and docetaxela) bind MD-2 and consequently inhibit LPS signalling. We also showed the most probable MD-2 - taxane binding modes and compared it with lipid binding.
COBISS.SI-ID: 4065818
The antimicrobial peptide PFWRIRIRR was produced in bacteria in the form of inclusion bodies. The solution structure of the peptide in SDS and DPC micelles is characterised by a well-defined short alpha-helix. The position of basic and apolar side chains indicates its positioning in the membrane that is also in agreement with characteristics of the peptide.
COBISS.SI-ID: 4071962
TLR4 receptor recognizes bacterial toxin LPS and is one of the most researched receptors, which plays an important role in many physiological processes. So far, the molecular mechanism of activation of this receptor has not yet been known. The above mentioned model representsnew paradigm of recognition and is important for devising new inhibitors and activation of TLR4
COBISS.SI-ID: 4120602
TLRs are type I transmembrane proteins. Up to now we only had structural information or models of separate domains of TLRs and much less on their required flexibility. Insertion of a linker between the transmembrane and ectodomain or within the ectodomain decreased activation. This suggests the requirement for tight coupling of the ectodomain to the membrane, which may facilitate its interaction with ligand, promote dimerization and prevent interaction with the cell-membrane surface.
COBISS.SI-ID: 4118298
The main function of the innate immune system from insects to mammals is to detect the presence of and act against invading microorganisms by recognizing their unique molecular signatures, most importantly, components of bacterial cell walls. A large number of peptides and derivatives, both synthetic and of natural origin, are known to influence immune responses in mammals by interacting with the conserved microbial structures, making the former attractive targets for drug development.
COBISS.SI-ID: 3785754