In this paper we compared electropermeabilization and electrofusion of two cell lines. Results were detected by counting nuclea in the cells. The method, developed for fusion of model cell lines were then used for fusion of mouse mieloma cells and human lymphocytes B. Heterohybridomas were prepared.
It is known that hypotonic fusion buffer improves electrofusion efficiency. We determined that different cell lines differ in their tolerance for incubation in hipoosmolar conditions. Swelling dynamics must be therefore determined for every cell line. The duration of increased cell volume is prolonged by electroporation and can improve electrofusion efficiency. Viability of cells after incubation in hypotonic buffer was not affected.For determination of electroporation parameters for electroporation of cells in hypotonic buffer, swelling of the cells must be taken into account.