The effects of cinnamaldehyde versus sodium monensin on growth, cell size and cell protein production in Prevotella bryantii B14 and Prevotella ruminicola 23 were studied. B14 strain was visibly more resistant to the action of both compounds. IC50 concentrations of sodium monensin influenced the increase in cell size of both bacterial strains during growth, but more pronounced in strain B14. A similar effect was observed cinnamaldehyde was used, indicating a possible interference with cell division. The action of cinnamaldehyde was highly concentration dependant in strain B14, but not in 23.
COBISS.SI-ID: 2365832
We described the nucA gene, whose product belongs to exonuclease/endonuclease/phosphatase Pfam family, and is involved in the removal of misincorporated uracil from DNA and its reuse. nucA codes for a signal peptide and, based on our results, confers non-specific DNase activity but no AP endonuclease activity, which is required for DNA repair subsequent to base excision. On the other hand, nucA homologues in related organisms from the phylum Bacteroidetes also code for signal peptides. It appears therefore that this feature is conserved and is not a peculiarity limited to P. bryantii.
COBISS.SI-ID: 2278280