Extraction of Se species, its separation with ion-exchange chromatography and ICP-MS detection was optimised and applied in the analysis of three commercial coenzyme Q10 based food supplements. Since in one product neither total Se content neither Se species present matched declaration, the possibility of reactions taking place among the different components present in supplements was studied. But, no transformation of Se species was observed. Additionally, in all products 25-45% of Se stayed insoluble. Therefore, the question arises, are antioxidant food supplements containing Se safe?
B.03 Paper at an international scientific conference
COBISS.SI-ID: 22460711The aim of our work was to find out how foliar addition of selenium affects the content of this element in buckwheat (Fagupyrum esculentum) and which Se species are present in seeds and leaves. Plants were sprayed with Na2O4Se (10 mgSe/L). Total selenium content was analyzed by HG-AFS, speciation was made using HPLC-UV-HG-AFS. Control group contained 5-45 ngSe/gDM, in treated group it was 50- to 500-fold higher. We observed similar selenium distribution in plant parts with or without Se. There was 60% of soluble Se in seed enzymatic extracts (95% in form of SeMet) and only 12% in leaves.
E.01 National awards
COBISS.SI-ID: 22192935The aim of this work was to study Se accumulation and to identify the Se species in potato (Solanum tuberosum L.) cultivar Desiree, enriched in Se by foliar fertilisation (10 mg Na2SeO4/L). Four combinations of treatments were conduced: well-watered plants with and without Se foliar spraying, and drought exposed plants with and without Se foliar spraying. The Se content was found to be less than 117 ng/g in non-treated potato, 300 ng/g in drought and 1000 ng/g in well-watered Se treated potato. SeMet and selenate were the main species in potato, regardless of the growth conditions (30% each).
B.03 Paper at an international scientific conference
COBISS.SI-ID: 21133607The aim was to evaluate the effects of foliar aplication of Na2Se04 on biochemical and physiological responses of chicory- varieties of 'Monivip' and 'Anivip', dandelion, salad rocket and wild rocket. Plants were sprayed twice with Se solution of (1 + 1; 2 + 2 and 5 +5 mg Se L-' and 10 + 0; 10 + 10 and 10 + 50 mg Se/L). In all varieties of leafy vegetables irrespective of the concentration of Na2Se04 solution, Se(V1) and SeMet were the most frequently present Se species in plants. Excessive amount of Na2Se04 caused a decrease in the non-enzymatic antioxidants, and higher ETS activity.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 6057081As a test plant we've chosen buckwheat (Fagopyrum esculentum Moench, cv. Darja). At flowering phase one group of plants was sprayed with 10 mgSe(VI)/L solution. Plants were air dried, separated to stems, leaves, inflorescences, dehusked ripe seeds and husks, then milled. Total Se content was analyzed by HG-AFS and enzymatic extracts speciation was made using HPLC-UV-HG-AFS. The main Se species found in seeds was SeMet (~60 % according to total Se content), while in stems, leaves and inflorescences the only form of soluble Se present was Se(VI). In husks no selenium species were detected.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 22228263