Four combinations of treatments were utilised: well watered plants with and without Se foliar spraying, and drought exposed plants with and without Se foliar spraying. Water-soluble Se compounds were extracted from potato tubers by water or by enzymatic hydrolysis with the enzyme protease XIV or amylase or a combination of protease XIV and amylase. Extraction was performed using incubation (37°C, 24h) and by ultrasound assisted extraction (300 W), using different extraction times. Se(VI) and SeMet were the main soluble Se species in potato tubers, regardless of the growth conditions.
COBISS.SI-ID: 5694329
Buckwheat (Fagopyrum esculentum), grown from seeds soaked in water, sodium selenate or sodium selenite (5, 10 or 20 mg SeIV/L) was exposed to reduced UV-B radiation, ambient, or enhanced UV-B. The mass fraction of Se in leaves was much higher in plants obtained from seeds soaked with Se(VI) in comparison to Se(IV). In buckwheat leaves 44.5–63.6 mg/100 g d.m. of fagopyrin was found, and in stems 14.3– 26.4 mg/100 g d.m.; here no influence of seed soaking solution or UV-B exposure was found. The content of total flavonoids in leaves was 7.8–15.9% and in stems 1.4–4.1%.
COBISS.SI-ID: 2270577
St. John’s wort (Hypericum perforatum L.) was grown under different levels of UV-B radiation, without and with Se foliar application (10 mg Na2SeO4/L). The different levels of UV-B radiation comprised an enhanced level simulating 17% ozone depletion, ambient level, and a reduced level of UV-B radiation. The concentration of Se in the organs of plants foliarly sprayed with Se ranged from 1000 ng/g to 12,000 ng/g, the highest concentration being detected in plants grown under reduced levels of UV-B radiation.
COBISS.SI-ID: 5952121
Buckwheat was foliarly sprayed with a water solution containing 10 mgSe(VI)/l at the beginning of flowering. Se content in the Se-sprayed group it was 50- to 500- fold higher, depending on the plant part. We observed a similar distribution of Se in plant parts in both control and treated groups, with the highest difference in Se content in ripe seeds. The main Se species found in seeds was SeMet (~60% according to total Se content), while in stems, leaves and inflorescences the only form of soluble Se present was Se(VI) (up to 10% of total Se). In husks no selenium species were detected.
COBISS.SI-ID: 6165625
The aim of this work was to check if commercially available enzymes are pure enough to be used for selenium speciation analysis and what contribution could impurities have on Se determination on a real sample basis. For this purpose, twelve commercially available enzymes of different origin and classification (protease, amylase, cellulase, lipase) were analysed. After the dissolution of the enzyme in water, separation of Se species was made by ion exchange chromatography, with ICP-MS used as the detection system. The results showed that the Se content was found to be relevant in several cases.
COBISS.SI-ID: 22198823