An increase in the intracellular free calcium concentration ([Ca2+]i) in astrocytes elicits a discharge of gliotransmitters from vesicles, probably by affecting vesicle trafficking. We showed that a rise in [Ca2+]i enhanced the speed of recycling VGLUT1 (vesicular glutamate transporter 1) vesicles and reduced the speed of ANP (atrial natriuretic peptide) vesicles. Cytoskeleton-severing agents abolished vesicle mobility, indicating a cytoskeleton dependent vesicle recycling. Our findings importantly contribute to the understanding of how vesicle mobility is regulated.
B.06 Other
COBISS.SI-ID: 25434329In the conference Trilateral Symposium of Physiology in honor of Helmut Hinghofer-Szalkay, Graz, Austria, September 18th - 19th 2008 the poster was presented about the method for labeling and monitoring of single vesicles in astrocytes was presented. The method was optimized on rat astrocytes. It enables specific labeling of vesicles, which fuse with the plasma membrane. During fusion vesicle components are exposed to the extracellular milieu; this enables binding of specific antibodies to label vesicles.
B.06 Other
COBISS.SI-ID: 24822233We presented preliminary data obtained by the new method for labeling and monitoring single vesicle transport in live astrocytes. Vesicles which fused with the plasma membrane and were retrieved back into the cytoplasm were labeled by antiboides against specific proteins in the vesicle lumen of the vesicle membrane. Transport of antibodies labeled vesicles which fused with the plasma membrane is for on order of magintude slower from the same types of vesicles which are trafficking form GA to the plasma membrane.
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COBISS.SI-ID: 24641241International Meeting Mechanism(s) of Exocytosis was held from May 22nd until May 25th 2008
B.01 Organiser of a scientific meeting
COBISS.SI-ID: 24626137Lecture at international conference "FEPS 2009 – The physiology meeting" in Ljubljana: The important process of communication between astrocytes and neurons is exocytotic release of gliotransmitters from vesicles into the extracellular space. We found that glutamate triggers vesicular ATP release from astrocytes and that ATP is found also in peptide vesicles. We described that peptide and glutamate vesicle traffic is cytoskeleton dependent and differently regulated by calcium ions.
B.06 Other
COBISS.SI-ID: 26396633