With new and rapid genotyping techniques, MIRU-VNTR, 121 previously RFLP typed M. avium isolates of the subsp. avium (Maa) and hominissuis (Mah) were analyzed to compare the discriminatory power of both methods and to find if the selected markers were suitable for the M. avium typing from a limited geographic area. This was the first MIRU-VNTR typing of Maa field isolates. A relative heterogeneity of Mah isolates was discovered in comparison to homogeneity of Maa when using MIRU-VNTR, therefore further investigations are necessary to develop a suitable MIRU-VNTR typing scheme for Maa.
MAP was inoculated into the compost in three actively ventilated and isolated vessels and in a conventional manure storage pile. The presence of MAP growth and MAP DNA was investigated with culture and IS900 PCR. Moisture, ash and ammonia content in the compost and manure specimens were determined and pH was measured. The results showed that manure composting can eradicate or at least drastically reduce the number of pathogenic microorganisms, and therefore it can be used as a preventive measure against spreading of MAP into the environment.
A review paper is treating diagnostic pathology of ulcerative colitis, Crohn's disease and indeterminate syndrome. Complications and pathogenesis are also summarized. Differential diagnoses are treated extensively.
Mycobacterium avium subsp. paratuberculosis (Map) has been the most enduring infectious candidate to be proposed as a causative agent of Crohn’s disease (CD), since it causes a very similar disease in animals, including primates. For the presence of Map, the archival formalin-fixed, paraffin-embedded intestinal tissues of CD Slovenian patients and control specimens of individuals without CD were tested by IS900 PCR. Map was detected in 12 % of the CD patients and none of the controls. Our findings contribute to the current knowledge about Map being a possible etiological agent of CD.
Seroprevalence of paratuberculosis in cattle herds in Slovenia was estimated. Animals older than two years in 20 % of herds of Slovenia were tested in 2008. A total of 38,374 sera from 6,780 cattle herds were examined in ELISA test. A total of 228 (0.59 %) animals from 188 (2.77 %) herds were positive. Currently, the seroprevalence of paratuberculosis in cattle herds in Slovenia is almost the same as it was in 1997 (2.77 % vs. 2.84 %). Compared to the majority of European countries, the herd prevalence is rather low. This could be partly attributed to a small number of animals per herd.