MAP was inoculated into the compost in three actively ventilated and isolated vessels and in a conventional manure storage pile. The presence of MAP growth and MAP DNA was investigated with culture and IS900 PCR. Moisture, ash and ammonia content in the compost and manure specimens were determined and pH was measured. The results showed that manure composting can eradicate or at least drastically reduce the number of pathogenic microorganisms, and therefore it can be used as a preventive measure against spreading of MAP into the environment.
Mycobacterium avium subsp. paratuberculosis (Map) has been the most enduring infectious candidate to be proposed as a causative agent of Crohn’s disease (CD), since it causes a very similar disease in animals, including primates. For the presence of Map, the archival formalin-fixed, paraffin-embedded intestinal tissues of CD Slovenian patients and control specimens of individuals without CD were tested by IS900 PCR. Map was detected in 12 % of the CD patients and none of the controls. Our findings contribute to the current knowledge about Map being a possible etiological agent of CD.