The overcome of endothelial barrier is a serious challenge in the treatment of brain tumors. Possible solution is delivery of drugs in liposomal carriers of adequate composition. For this purpose liposomes of different composition were prepared and influence of membrane fluidity on the uptake and transcytosis by endothelial (MDCK) cells, a model for blood brain barrier, investigated. The most pronounced transcythosis was obtained for liposomes composed of DPPC, cholesterol, DOPC and 20 mol% alkylphospholipid that experienced the highest membrane fluidity among the liposomes investigated
B.03 Paper at an international scientific conferenceCOBISS.SI-ID: 22514727
For many investigations on cells and interactions of biologically active substances with cells detachment from the bottom of the culture dishes is necessary. However, detachment can cause severe damage to cells and influence the reliability of the results. According to our results, among the detachment procedures used (scraping cells, removal by citrate buffer or trypsin) the most suitable seems to be trypsinistation, which influences cell viability and metabolism of cells the least, while neither of the methods influence membrane fluidity significantly.
B.03 Paper at an international scientific conferenceCOBISS.SI-ID: 22514983
Role of membrane domain structure in the process of transcytosis across the blood brain barrier (BBB) means a new scientific knowledge which will help in understanding the mechanisms of transcytosis and consequently in the preparation of new better delivery systems for transport of drugs through BBB: In the research young scientists were included, who earned new practical knowledge and experience which will help in their future work.
B.03 Paper at an international scientific conferenceCOBISS.SI-ID: 23043111
To get better insight into the mechanism of transport of OPP through the cell membrane the spin labeled OPP (5P) was used and the kinetics of transport of free 5P and 5P in liposome formulation into the model and cell membranes was investigated by EPR. We have found that the transport of 5P from liposome membrane into the membrane of other liposomes or cells was fast (equilibrium was reached within few min), while the transport from outer layer to the inner layers of the membrane is a slow process. Therefore we propose 5P as a good spin probe for the study of model and cell membranes.
B.03 Paper at an international scientific conferenceCOBISS.SI-ID: 24379431
Role of membrane domain structure in the interaction of liposomes with cell means a new scientific knowledge which will help in understanding of cell membrane interactions. In the study young scientists were included, who earned new practical knowledge and experience which will help in their future work.
F.01 Acquisition of new practical knowledge, information and skillsCOBISS.SI-ID: 22164007