For many investigations on cells and interactions of biologically active substances with cells detachment from the bottom of the culture dishes is necessary. However, detachment can cause severe damage to cells and influence the reliability of the results. According to our results, among the detachment procedures used (scraping cells, removal by citrate buffer or trypsin) the most suitable seems to be trypsinistation, which influences cell viability and metabolism of cells the least, while neither of the methods influence membrane fluidity significantly
B.03 Paper at an international scientific conferenceCOBISS.SI-ID: 22514983
Role of membrane domain structure in the process of transcytosis across the blood brain barrier (BBB) means a new scientific knowledge which will help in understanding the mechanisms of transcytosis and consequently in the preparation of new better delivery systems for transport of drugs through BBB: In the research young scientists were included, who earned new practical knowledge and experience which will help in their future work.
F.02 Acquisition of new scientific knowledgeCOBISS.SI-ID: 23043111