The article describes the results of biochemical study, where we demonstrated and validated inhibitory activities of the selected n-hCCP against some of serine and lysosomal cysteine proteases. Also some of their effects on normal and tumour cell line phenotype characteristics, such as viablitiy and proliferation are described.
This work is the first experimental proof and demonstration of the existence of prokaryotic cyatoskeleton. Also, the role of lysogeny during the caynobacterial bloom lysis and experimental evidence of the presence and formation of focal point, is presented. Oxford Univ. Press have decided to put the image from this article on the cover page of November issue of Vol. 31, No. 11 and the article was ranked as the first among the presented scientific papers.
In this contribution we described, along with the first author form the group of Rolf Bjerkvig, collaborating with many European laboratories, on the observation of MSC transformation. This has been previously described as spontaneous , but here we reported that this was not the case, but in fact the consequence of contamination with the tumour cells, kept in these laboratories. This is an important message warning against false results, when cells are not regularly tested by DNA fingerprinting to assure their identity.
During cell lysis are cyanobacterial products, mainly confined to the interior of the cells, massively released. Therefore this phenomenon is the most critical moment for human and environmental health. We have proposed a new mechanism of cyanobacterial population control, where temperate viruses, that allow extensive proliferation of the most competitive species, play the central role. This theory explains also the high frequency of non-toxic blooms and the variability of bioactive CCP’s production even in almost mono-specific blooms
We develped the visualisation of the cyanobacterial cytoskeletal framework using anti-bovine alpha-tubulin mouse monoclonal antibodies on Microcystis aeruginosa cell lines in vitro and on various cyanobacterial bloom samples from nature. This method allows us to pursue cyanobacterial bloom lysis, to identify already damaged and lysed cyanobacterial genera or even species along with other phytoplankton. Additionally we can discriminate between healthy and senescent organisms, and by visualisation of the division ring the proliferation potential of cyanobacteria.