In October and November 2009 Uroš Petrovič, a member of the project time, visited the laboratory if Igor Stagljar, who is the founder of the membrane yeast two-hybrid method, at the University of Toronto, ON, Canada. Te purpose of the visit was to learn the technique and apply it in the identification of protein-protein interactors of Izh2 protein, a yeast homolog of the adiponectin receptor. Ten potential interactors have been identified.
B.05 Guest lecturer at an institute/university
COBISS.SI-ID: 2073200To minimize the amount of required substances to be tested in the genome-wide screening, a higher density yeast colony array was developed. We upgraded the custom-made robotic manipulator to perform pinning at 1536 colonies/plate (previously 384). Currently used methods measure yeast colony area, which however does not take into account differences in three-dimensional shapes of the colonies. We developed an approach whereby we obtain information on the 3D shape by transmission scanning and using a dedicated algorithm to determine the volume.
F.19 Knowledge that leads to a company spin off
COBISS.SI-ID: 2073200