The yeast Saccharomyces cerevisiae is an important host for production of a variety of heterologous proteins that have been used for the production of therapeutic and diagnostic agents, and as vaccines. The salient feature of this invention is the replacement of the GAL1 gene with the GAL4 gene in the chromosome of the yeast Saccharomyces cerevisiae. The locus of the GAL1 gene encoding the galactokinase enzyme is, by means of homologous recombination, replaced with the gene for the Gal4 transcription activator. The designed gene replacement causes in the mutant S. cerevisiae strain a significantly improved synthesis of recombinant proteins, expressed under the control of the GAL1 promoter. Highly productive recombinant S. cerevisiae strains resulting from the GAL genes replacement in the genome are applicable in the expression of industrially (pharmaceutically) interesting proteins. By the utilization of the recombinant strain, which is an object of this invention, the inputs are significantly diminished (galactose is a relatively expensive inductor), and simultaneously a high level production of the desired recombinant protein is achieved.
F.32 International patent
COBISS.SI-ID: 28682713DiaGenomi Ltd is a young high-tech start-up company that was founded by two young researchers (dr. Klemen Španinger in dr. Rok Košir), both being the programme P1-0104 (MCMB MF) members, who after completing their theses became employed in the company. The company received the following awards in 2011: - Best business plan 2011 - Technology park Ljubljana. - Best business plan 2011 - Start-up Slovenia. - Best Start-up company 2011 - Start-up Slovenia. - Runners up - Podjetna Primorska 2011. - Finalists of Deans award 2011. - Finalists UNICA Entrepreneurship competition 2011. - Participants of the 6th Slovenian forum of Innovations. The company has repeatedly appeared in different forms of media (print, radio, Internet and television). Dr. Klemen Španinger was also an individual winner (beside Véronique Bos, NL) of the 1st Regional Novartis International Biotechnology Leadership Camp (BioCamp) Alpe Adria, Ljubljana, Slovenia, 29 – 31 May 2011: http://www.lek.si/si/o-nas/mediji/sporocila-za-javnost/665/ [proposer: R. Komel].
F.20 Company spin off
COBISS.SI-ID: 29074393A college text-book covering all required contents, enriched with relevant illustrations from the everyday life and with links to the real world, in order to falicitate understanding of the abstract concepts and terms. It has two graphically separated parts, Cell Biology and Genetics. The last two pages of each book chapter are shaped as extra folders in which on the left side one can find summarized "Key comprehensions of the chapter", and on the right side a column "Use your knowledge". At the end there is a dictionary with explanations of the key concepts/terms present throughout the text-book, as well as answers to questions from individual chapters.
D.10 Educational activities
COBISS.SI-ID: 254618112