Composite dietetic milk draught and method for production of composite dietetic milk draught with reduced level of uric acid and purine compounds is designed with the aim to eliminate uric acid, as well as purine nad pyrimidine nucleotides, deoxyribonucleotides, nucleosides and free bases (adenine and guanine), which are naturaly present in milk. Charcoal is used as a method for adsorption, which implicates irreversible binding of uric acid and purine derivatives from milk. The additional benefit is the binding of heavy metals and lipids, what makes positive effect on proposed dietetic product, since heavy metals contribute to tubule damage and aggravate uric acid secretion at the kidney tubule level, while hypelipidemia nad hyperuricemia commonly appear in some diseases. Technological procedure for production of proposed dietetic formula proceeds through the following phases: collection of primary milk sample from milk dairy, uric acid and purine separation by charcoal adding in proposed dose, milk sample homogenisation, milk fat standardisation to 0.5%, charcoal elimination by centrifugation and filtration. Supplementary addition of all or individual compound (Vitamin C-L-ascorbic acid in a dose of 1000 mg/L, Coenzyme Q10 of natural origin in a dose of 200 mg/L and L-Arginine in a dose of 500 mg/L), sample deodurisation, UHT sterilisation and milk packaging.
F.33 Slovenian patent
COBISS.SI-ID: 3139372Tylosema esculentum (marama) beans and tubers are used as food, and traditional medicine against diarrhoea in Southern Africa. Rotaviruses (RVs) are a major cause of diarrhoea among infants, young children, immunocompromised people, and domesticated animals. Our work is first to determine anti-RV activity of marama bean and tuber ethanol and water extracts; in this case on intestinal enterocyte cells of human infant (H4), adult pig (CLAB) and adult bovine (CIEB) origin. Marama cotyledon ethanolic extract (MCE) and cotyledon water extract (MCW) without RV were not cytotoxic to all cells tested, while seed coat and tuber extracts showed variable levels of cytotoxicity. Marama cotyledon ethanolic and water extracts (MCE and MCW, resp.) ()= 0.1 mg/mL), seed coat extract (MSCE) and seed coat water extract (MSCW) (0.01 to 0.001 mg/mL), especially ethanolic, significantly increased cell survival and enhanced survival to cytopathic effects of RV by at least 100% after in vitro co- and pre-incubation treatments. All marama extracts used significantly enhanced nitric oxide release from H4 cells and enhanced TER (Omega/cm(2)) of enterocyte barriers after coincubation with RV. Marama cotyledon and seed coat extracts inhibited virion infectivity possibly through interference with replication due to accumulation of nitric oxide. Marama extracts are therefore promising microbicides against RV.
F.06 Development of a new product
COBISS.SI-ID: 3094572The Old Vine from Lent (Maribor, Slovenia) which belongs to the 'Modra Kavcina' group (i.e. 'Blauer Kolner' in the Vitis International Variety Catalogue and 'Zametovka' in the official varietal list of Slovenia) is considered to be the oldest living specimen of cultivated grapevine (at least 400 years old). The aim of our study was to determine the genetic relationships among different accessions of the 'Zametovka' group, the position the Old Vine within this group, and the relationship between the Old Vine and other red varieties grown in Slovenia and neighbouring countries. The molecular genetic analysis was based on microsatellite data. The study shows that the 'Zametovka' group is genetically completely different from other red varieties studied. Among these genetically distant varieties, in our study, 'Chasselas red' appears to be the closest. The 'Zametovka' group is genetically highly homogenous, and hall of the studied accessions probably belong to the same clone. The 'Old vine' cannot be considered as a significantly different genotype. The minor differences detected by microsatellite markers are probably due to mutations accumulated over a long period of time and possibly to epigenetic changes.
F.27 Contribution to preserving/protecting natural and cultural heritage
COBISS.SI-ID: 3206956As the nutritional quality of food is becoming increasingly more important forconsumers, significant attention needs to be devoted to agricultural practices and their influences on the nutrient contents in food. The presentedinvestigation studied the mineral contents and antioxidant activitiesin the fruits of four organically-grown strawberry cultivars 'St. Pierre', 'Elsanta', 'Sugar Lia' and 'Thuchampion' when compared to those of integrated-grown plants. The strawberries were digested and analyzed for K, Mg, Fe, Zn, Cu, and Mn using an atomic absorption spectrometer, whilst P was analyzed using a vanadate-molybdate method. In addition, antioxidant activity was estimated by using the ABTS assay. The results showed that the mineral contents and antioxidant activities in strawberries depends on the cultivar, and its production system. Organically-grown fruits showed higher antioxidant activities and Cu content than the integrated fruits, whilst the integrated fruits were superior in their contents of P, K, Mg, Fe and Mn. All the cultivars showed similar Zn content, probably reflecting the fact that the Zn content in strawberries does not depend on the cultivar.
F.14 Improvements to existing production methods and tools or processes
COBISS.SI-ID: 3490604Cencič, Avrelija (editorial board member 2002-)(Print ed.). Amsterdam: Elsevier. ISSN 0168-1605. The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interst, and is of high scientific quality. The journal has impact factor of 3.425.
C.04 Editorial board of an international magazine