We monitored binding of EqtII-GFP to cell membranes and observed binding of EqtII-GFP to membranes of Golgi apparatus when expressed intracellularly, or binding to apical membranes of MDCK cells when applied extracellularly; EqtII-GFP did not bind to basal membranes that do not contain sphingomyelin. EqtII-GFP performed as a sensitive probe, which enables further research of its physiological role.
Communities of endophytic fungi on the roots of goat willows (Salix caprea) growing in soil with different degrees of heavy metal pollution, were analysed using molecular tools. Additionally, leaf accumulated metals and photosynthetic pigments were analysed for evaluation of the fitness of goat willows growing at the polluted site.
Nuclear ribosomal sequences and Cd, Zn, Pb and Fe accumulation of different populations of hyperaccumulating species Thlaspi praecox Wulfen (Noccaea) were studied to reveal their relationships to other representatives of the genus and especially to the well known hyperaccumulator T. caerulescens; comparisons of their accumulating properties were also made.
St. John’s wort, grown under different levels of UV-B radiation, was analysed for its concentrations of flavonoids, tannins and hypericin. A high level of UV-B radiation increased leaf concentrations of flavonoids and of tannins. The concentration of hypericin in leaves exposed to enhanced UV-B radiation was lower than that of leaves exposed to reduced UV-B radiation.
High amounts of Cd were measured in seeds without drastically affecting seed viability. In intact seeds and isolated embryos, almost two thirds of the Cd ligands were thiol groups (Cd-S-C-) while in roots and shoots up to 80% of the Cd ligands were oxygen ligands that are provided by the cell walls and by organic acids stored in vacuoles.