The review article is a comprehensive review of preclinical and clinical studies on IL-12 electrogene therapy of cancer. The preclinical studies have demonstrated that IL-12 gene elctrotransfer is an effective approach as local or systemic treatment. Translation of preclinical studies into clinical trials in human and veterinary oncology has started with encouraging results that would hopefully lead to further investigation of this therapy, also in combination with other cancer treatment modalities.
COBISS.SI-ID: 980859
Electroporation and electrochemotherapy induce a profound reduction in tumor blood flow, which contributes to the antitumor effect. The antitumor effectiveness of electrochemotherapy has been proven extensively in the treatment of malignant melanoma, and now also exploited for palliative treatment of bleeding melanoma metastases. It demonstrated immediate cessation of bleeding and very good antitumor effectiveness which is of utmost importance for translation of electrochemotherapy into the treatment of deep-seated tumors, especially in well vascularized organs.
COBISS.SI-ID: 929659
Based on the in vitro selection of siRNA molecules against oncogene k-Ras, we were the first to prepare plasmid DNA encoding miRNA against K-ras gene. In experimental tumor model of human colorectal carcinoma in mice we demonstrated silencing of the target gene and also effectiveness on tumor growth. As transfection method we used electroporation.
COBISS.SI-ID: 923771
Magnetic nanoparticles are examined as delivery systems for different molecules. The aim of our study was to evaluate physicochemical properties, cytotoxicity, cellular uptake and trafficking pathways of the custom-synthesized magnetic nanoparticles. We managed to prepare in isotonic solution stable magnetic nanoparticles with a diameter of 10 nm, which were not cytotoxic in a broad range of concentrations. They were readily internalized into different cells, however exposure to magnetic field increased their cellular uptake, predominantly into malignant cells.
COBISS.SI-ID: 967035
In the paper, on one of the largest series of atypical and malignant meningiomas discovered pathohystological and clinical predictors (mitotic count, brain invasion location and Ki-67) for recurrence free survival despite surgical excision were discovered. This factors than influence further therapeutic strategy of atypical and malignant meningiomas
COBISS.SI-ID: 512126777