To asses the therapeutic potential of autologous myoblasts in treatment of stress urinary incontinence (SUI) myoblasts were isolated from a biceps muscle sample and injected under transurethral ultrasound guidance into the external urethral sphincter of 38 female patients. The patients also underwent electric stimulation postoperatively to enhance cell integration. No serious adverse events or complications were noted and the procedure was well tolerated. The results to the stress test were negative for 29 (78.4%) of the patients, 5 (13.5%) considered their SUI cured, and 29 (78.4%) reported improvement.
B.06 Other
To evaluate the presence of stem cells in the ovarian surface epithelium of patients with premature ovarian failure and no mature follicles and oocytes we observed small round cells of unknown origin expressing SOX-2 marker of pluripotency. These cells were an integral part of the ovarian surface epithelium. When the scraped cells were cultured in a medium with added follicular fluid to provide some ovarian niche, primitive oocyte-like cells and typical round-shaped cell clusters positively stained on alkaline phosphatase, and markers of pluripotency, such as SOX-2 and SSEA-4, were developed. Single oocyte-like cells expressed genes OCT4A, SOX-2, NANOG, NANOS, STELLA, CD9, LIN28, KLF4, GDF3, and MYC, characteristic for pluripotent stem cells. The results of this study confirmed the presence of putative stem cells in the ovarian surface epithelium of these patients and provided some basis to create a stem cell line in the future.
B.06 Other