Chronic peroral inoculation with periodontal pathogen Porphyromonas gingivalis induces systemic immune response as determined by nitric oxide formation in internal organs and elevated levels of its' metabolites nitrite and nitrate and TNF-a in plasma of mice before any periodontal disease is observed. The fact, that the whole body reacts to the entering periodontal pathogen in early phases of infection, could add to the current understanding of pathogenesis and prevention of periodontal disease, and modulation of interaction between host immune response and antigen in humans and animals.
COBISS.SI-ID: 3055482
We evaluated antitumor effect of IL-12 EGT on murine SA-1 sarcoma. The IL-12 plasmid was applied either intra- or peritumorally. Both approaches resulted in remarkable local and systemic antitumor effect with significant intratumoral and systemic production of IL-12 and IFN-?. Intratumoral EGT resulted in 90% complete response rate to treatment with 60% resistance to challenge with SA-1 tumor cells. Peritumoral EGT resulted in significant growth delay of treated tumors with 16% complete response rate. Furthermore, significant growth delay of untreated tumors at a distant site was achieved.
COBISS.SI-ID: 3131770
Serum leptin concentrations were measured in 107 Lipizzan fillies aged 1 to 4 years from January to December. Values in 3- and 4-year olds were higher than in 1 and 2-year olds (P(0.001). Leptin levels in 3 and 4-year olds peaked in August and declined during autumn (P(0.001). The highest leptin level was in fillies exhibiting continuous and the lowest in those, exhibiting seasonal cyclicity (P ( 0.001). In anoestrous fillies leptin level was lower than in fillies with prolonged or whole-year cyclicity but higher than in fillies with seasonal cyclicity (P)0.05).
COBISS.SI-ID: 2846074
We developed a BRET-based screening assay specifically tailored for the CB1R, which is an important target in the field of obesity. CBIR interacts weakly with the b-arr2 and hence performed unsatisfactory in our existing screening platform. To enhance its ß-arr2 binding capacity, we replaced the C-terminal tail of the CB1R with tails from either the V2 or BRS3 receptors, both of which interact strongly with ß-arr2. Using this chimeric approach, a small compound library was screened and 21 antagonist and inverse agonist hits identified with IC50 and EC50 values ranging from 0.3 nM to 7.5 µM.
COBISS.SI-ID: 3060346
On the basis of the enzyme-immunohistochemical properties of the skeletal muscle fibres, we assume that MHC-I, -IIa and -IIx isoforms are expressed in the muscles of adolescent bears, however in adult, full-grown animal, only MHC-I and -IIa isoforms are contained. MHC-I isoform is expressed in slow fibres and co-expressed with the fast, presumably -IIa isoform in the hybrid fibres. High oxidative and weak glycolytic capacity was established in all muscle fibres of bear. The expression of one slow and two fast MHC isoforms obviously fulfils extraordinary locomotor capacity of this species.
COBISS.SI-ID: 3031930