Using 2D electrophoresis, we compared proteomic profile of biceps femoris muscle (insoluble fraction) according to genotype (PRKAG3Ile199Val and CASTLys249Arg/Ser638Arg), as well as salt content and pastiness. The gene PRKAG3 affected mainly metabolic enzymes. The impact of the CAST gene was smaller compared to PRKAG3, however, we could identify a link with fragments of actin. Salt content and pastiness were associated with metabolic and myofibrillar proteins, which can be ascribed to differences in proteolysis. Significant differences between proteomic profile based on pastiness and salt content indicates the possibility to search for potential markers of quality at the proteomic level.
COBISS.SI-ID: 3517032
The genus Cosmospora is revised according to molecular sequence analyses and interpretations based on morphological characters. It is shown that the genus Fusarium is to be segregated into two large groups, of which one accommodates Fusarium sensu stricto. The other group accommodates several genera such as Microcera, Macroconia, Dialonectria, Fusicolla, Stylonectria reconsidered distinct from Fusarium. Species of Microcera have a potential as biocontrol agents against important armoured scale insects on fruit trees.
COBISS.SI-ID: 3565160
A sampling approach, a five steps system for monitoring the adventitious presence of GMOs in the field was developed. Fitting function, which parameters are estimated in the receptor field at the two distances from the donor field, is used along with the field lenght to determine the presence of GMOs in the maize field. The worldwide importance of the developed approach lies in its applicability to address other threshold levels beside the currently prescribed labelling threshold of 0.9% for food and feed in the EU.
COBISS.SI-ID: 3210344
A zymography technique, applied to aminopeptidases and peptidases in crude bean leaf extracts, based on native polyacrylamide gel electrophoresis (PAGE) has been devised, which enables the substrate specificities, content and pH profiles of proteolytic enzymes to be determined in an unfractionated tissue extract. One enzyme active against Ala–AMC (7amino4methylcoumarin),one enzyme active against ZArg–AMC, several enzymes active against Leu–AMC, and (for the first time in plants) several enzymes active against Phe–AMC were identified.
COBISS.SI-ID: 3576952
About 250 bumblebee species in 15 subgenera are known in the world. Identificantion of some species is difficult due to small morphological differences. In this study wing venation pattern was analyzed and obtained characters were used for species identification. 469 bumblebees from 121 localities in Slovenia and 61 imported individuals were included in the analyses. The coordinates of 19 vein crosses on the forewings were measured and used in the calculation of 37 wing characters. Based on discriminate method more than 97 % of all analysed bumblebees were assigned to the correct species. The most informative characters were angles J16, A4 and discoidal shift. Wing venation pattern also differed between autochtomous and imported Bombus terrestris individuals.
COBISS.SI-ID: 3630696