A bioinformatics data mining approach was applied for the development of new hop genic microsatellite markers derived from public cDNA and EST sequences. Seventy-two microsatellite-containing sequences were discovered in public hop sequences. Eleven additional sequences were isolated from the enriched library. Thirty four primers were developed and 27 yielded successful amplification. Of these, 11 pairs amplified 12 loci, which were integrated to the existing microsatellite maps of hop and added new 38.6, 25.8 and 40.0 cM to the female, male and integral maps.
COBISS.SI-ID: 6353273
A method based on non-concerted evolution of the nrITS region was used in combination with genetic analysis of cpDNA variable regions to reveal the origin of horticultural groups within Allium ampeloprasum and the section Allium. Results showed that cultivated groups differ, with Great-headed Garlic being a complex allohexaploid or allooctoploid , while leek and other two cultivated varieties are autopolyploids and do not therefore belong to the same species. A correction of the taxonomic rank has been proposed and the results are useful for further hybridization attempts.
COBISS.SI-ID: 6122873
The gynogenic responsiveness of a second generation of double haploid onion lines showed slightly diminished gynogenic responsiveness and a genotype dependent response. A novel protocol based on in vitro culture of mature haploid ovaries and induced embryogenesis was established, aimed at inducing chromosome doubling. The method was found to be very efficient based on individual line criterion and this achievement provided the elimination of a significant bottleneck for DH protocol in onion.
COBISS.SI-ID: 6250105
The variety 'Rebula' is an indigenous an also ancient variety in the Slovenian and Italian world renowned winegrowing district Collio, dating back to the period of the Roman Empire. DNA analyses of the variety ‘Rebula’ and its potentially related varieties were conducted to evaluate its biodiversity. DNA analysis showed that deviations in synonyms and homonyms linked to the variety 'Rebula' still exist, which impedes its standardisation and revitalization.
COBISS.SI-ID: 6464889
Twenty-eight basil accessions were studied using molecular markers, nuclear DNA content and chromosome counting. The results supported the existence of several infrageneric groups within the genus and the section was further divided into two separate clades. The differences in genome size and chromosome number among Ocimum species indicate that evolution of their genomes was accompanied by both sequence deletion/amplification and chromosome rearrangements and polyploidization.
COBISS.SI-ID: 6214521