At the invitation of the Editors J. Murovec and B. Bohanec drafted a chapter in a textbook entitled "Plant Breeding". The reason for macceptance was the option that this textbook is offered to the readers on the Open access basis. In the chapter entitled " Haploids and Doubled Haploids in Plant Breeding " the authors describe the need to use haploid and doubled haploid lines in genetics and breeding. Procedures to achieve this goal are described in details: a) Induction from haploid female gametophyte - induction and in situ induction triggered with pollination with the pollen of the same species, closely related species or after irradiation, and induction achieved by growing unfertilized ovules, ovaries or flowers in vitro, b) androgenesis- in vitro induction from anthers or microspores. In other sections the following is described: options for ploidy level determination and homozygosity testing, procedures for duplication of chromosome number, applications of doubled haploids in plant breeding and novel approaches combining DHs and molecular genetics.
COBISS.SI-ID: 6956153
Diversity Arrays Technology is a micro-array based DNA marker technique for genome wide discovery and genotyping of genetic variation. An international consortium (Australia, USA, UK, Slovenia) screened 86 accessions of hop from Europe, North America (NA), Asia and Australia, using DArT. DArT identified 730 polymorphic markers and the markers differentiated between and within material of European, North American and Asian origin, with related accessions and accessions from a single cultivar known by several synonyms, cluster together. The reproducibility of DArT markers, the level of genetic variation detected with this system in hop and the resolution of known genetic affinities are positive signs that DArT provides the platform for future exploration of marker-trait associations to develop marker assisted selection technologies in hop.
COBISS.SI-ID: 6572153
Microsatellite genotyping was applied to a set of 38 local Slovenian varieties with an aim of their determination and characterization. In the analyses we used 11 SSR markers, which have been proven as highly polymorphic for genotyping of European Vitis resource. The markers have high PIC value of 0.766, with a total number of 98 alleles and a mean of 9.6 alleles per locus. High level of genetic diversity among local Slovenian grapevines was obtained and some unknown relationships among varieties were discovered. The use of reference cultivars made our results comparable to some previously reported allelic profiles. The identity analyses revealed 11 pairs/groups of synonyms and several varieties with similar names resulting in different SSR profiles. Through microsatellite profiling the identification of varieties was enabled which can facilitate the establishment of germplasm collection of old, local Slovene grapevines.
COBISS.SI-ID: 6646649