The work was presented as a plenary lecture at the 40. conference on yeasts in Smolenice, Slovakia. It describes the biotechnological value, biodiversity and safety issues of leading yeast Saccharomyces cerevisiae. During last decade we showed that this yeast is a good industrial, good model organism, but also an organism which might compromise health of humans. We showed a huge biodiversity of S. cerevisiae strains during wine fermentations and we are continuing to explore mechanisms of these interactions between cells and between other yeast species.
B.04 Guest lecture
COBISS.SI-ID: 4086392Academic achievements at the chair of biotechnology, microbiology and food safety in the past 20 years is presented in three papers, Educational and research cooperation is illustrated with 8 cases. Research cooperation is presented in 31 projects. List of BSC, MSC and Phd graduates from the chair of biotechnology, microbiology and food safety is well documented with statistics and carrier path. From 201 graduates is 35 PhDs
B.01 Organiser of a scientific meeting
COBISS.SI-ID: 263124736The chapter clearly defines standard ISO 22000 and integrates all relevant knowledge and skills regarding food safety in food supply chain.
B.06 Other
COBISS.SI-ID: 3945080This patent application describes the methods of biosynthetic engineering directed to produce novel medicinally important compounds of polyketide origin based on the chemobiosynthetic/mutasynthetic approach, where analogues of unnatural extender units are added into cultivation media of actinomycete cultures. These unnatural units are incorporated by polyketide synthases into polyketide backbones of compounds, such as the antibiotic erythromycin, thereby incorporating novel functional groups, which cannot be introduced by usual procedures of chemical semi-synthesis. In addition, these functional groups are chemically amenable and allow for subsequent derivatization of the obtained bioactive compounds. An important part of the patent application relates to production of novel erythromycin analogues and is based on the bioprocess, gene tools and transformation procedures, developed in Acies Biofor Saccharopolyspora erythraea as well as on new insights on this high-producing strain obtained by proteomic analyses.
F.09 Development of a new technological process or technology
COBISS.SI-ID: 4162936A lecture presented by the project leader (IMM) was chosen for oral presentation among 1700 abstract at the 14th ISME conference in Copenhagen, which is the most important and largest conference on microbial ecology in the world. The talk indicated quorum sensing (QS) as a paradigm mechanism of social behaviour in bacteria and the main achievements of this project were presented. This pertains to diversification of the QS system in relation to ecotypes. Using strains isolated from soil microscale we recently showed that (1) multiple ecotypes of B. subtilis coexist within the soil microscale and, most importantly, (2) that each pherotype found within this species, is largely, although not perfectly, associated with a single ecotype. These experimental findings were then used as a basis for two new models (pherotype cycling model and occasional advantage model) that propose to explain the origin and maintenance of this diversity and the advantage of occasional communication across ecotypes and even different species taxons. In addition, results on changes of QS response were observed in mixed populations. In biofilms the presence of the mutant in the wild type population increased the heterogeneity of the QS response, while the response of the mutant mixed with the wild type in the planktonic culture showed was higher in comparison to the wild type.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 4129912