A review of the prevalence and mechanisms of the multiple antimicrobial resistance of Campylobacter in the food chain is given, with the emphasis on non-specific efflux pumps which are involved in bacterial reduced susceptibility and/or resistance against antibiotics and other unrelated antimicrobials, e.g. bile salts. For the first time the synergistic antimicrobial activity of some plant compounds (e.g. EGCG) which acts as an inhibitor of the efflux pump CmeB is presented as a challenge to develop efficient protection against antibiotic and/or bile salt resistant Campylobacter strains.
COBISS.SI-ID: 3740280
The antioxidative activity of propolis extract (EEP) and its main phenolic compounds were investigated in the yeast Saccharomyces cerevisiae as a model organizem. After 1-h exposure of the yeast cells, their intracellular oxidation was measured, which decreased, but only in the case of propolis extract. However, cellular uptake was only seen for a moderate polar fraction of EEP and caffeic acid phenethyl ester (CAPE). The influence of EEP was also investigated at the mitochondrial proteome level, where changes in the levels of antioxidative proteins and proteins involved in ATP synthesis were seen.
COBISS.SI-ID: 3949176
This is a pioneering work published in the top journal (1/31) in the field of soil science, which presents novel findings on diversity of bacterial laccases in soil. The work demonstrates that laccases are wide spread in bacteria and may be more important than fungal laccases. A new primer was constructed to retrieve larger fragments of the putative bacterial laccase genes and importantly, the genes for both “conventional” 3-domain laccases and the recently described 2-domain small laccases were obtained, demonstrating a potential of the primer for discovering laccase-like sequences in bacterial strains, metagenomic libraries and in environmental samples.
COBISS.SI-ID: 3874680