In this work we taxonomically described and technologically evaluated two new yeast species named Candida adriatica. sp nov. and Candida molendinolei sp. nov. isolated from Slovenian extra virgin olive oil. After publishing the nucleotide sequence of rRNA gene regions in a public database scientists who isolated this yeasts from Croatian and Italian olive oil and by-products of its production joint our research. The two species were evaluated for their technological importance. Candida adriatica had a strong extracellular lipolytic activity, which proves that the species is a potential spoilage organism of olive oil.
COBISS.SI-ID: 4073592
Therapeutic use of macrolides in humans and animals is important in the selection of macrolide-resistant Campylobacter isolates. Using mutants in CmeB and CmeF efflux pump genes and CmeR transcriptional repressor gene, we confirmed efflux pumps as potential targets for development of therapeutic strategies with macrolides and efflux pump inhibitor (EPI), to restore the macrolide efficiency. Epigallocatechin gallate modifies Campylobacter multidrug efflux systems and restors the macrolide efficiency in resistant strains.
COBISS.SI-ID: 4119160
Anti-Campylobacter activities of plant phenolics and the role of bacterial drug efflux systems in the resistance to natural phenolics in C. jejuni were defined. The highest antibacterial activity was confirmed with carnosic and rosmarinic acids in their pure forms or in enriched plant extracts. The drug efflux systems in Campylobacter contribute significantly to its resistance to the phenolics and EPIs potentiate the anti-Campylobacter activities of plant phenolic compounds.
COBISS.SI-ID: 4160120
Within this paper, which was published in the top journal in the field of agricultural engineering (1/12) and with IF=4.98, we optimised the methodology for investigation of structure and genetic composition of bacterial community at the level of 16S rRNA in moving bed biofilm bioreactors (MBBRs) treating municipal wastewater (WW) or synthetic waste water. This work represent the bases for further testing of influence of pharmaceuticals or other environmental factors on biofilm community and provides the first evidence that influent type has a stronger influence on community structure than operational conditions, such as temperature or bioreactor size.
COBISS.SI-ID: 4041848
In this article we report the development of a novel bioprocess which enables exclusive biosynthesis/production of the immunosuppressant FK506 (tacrolimus) without simultaneous production of two structurally very similar compounds FK520 and dihydroFK506. Traditional bioprocesses for production of FK506 result in the levels of these two byproducts between 10 and 20% of the produced FK506, which due to great structural similarity of the compounds hampers the isolation of FK506 for use in pharmaceutical industry. Purification using industrial HPLC greatly increases the price of the final product as well as the environmental burden due to large amounts of organic solvents used. The novel process is based on the targeted genetic modification of the strain Streptomyces tsukubaensis NRRL18488 in which the supply of building blocks for polyketide synthase was abolished. Fermentation broths of the modified strain were then supplemented with a synthetic analogue of the allylmalonylCoA extender unit.
COBISS.SI-ID: 3988600