We introduced a quick and easy test for determining neuraminidase enzymatic activity (NEAC). We used a chromogen substrate BIN (B4666, Sigma) and with this test, we demonstrated for the first time that a pathogenic bacterium Ornithobacterium rhinotracheale (ORT) has NEAC. We also evidenced antibodies against MS and MG neuraminidase in chickens and turkeys infected with these two bacteria. What is more, approximately 25% of chickens that were infected with MS, had antibodies in sera that inhibited its NEAC. We also proved that NanH of MS cleave sialic acid from the chicken serum glycoproteins, including IgG, with SAα (2-6) gal moiety. NanH desialylates also the glycoproteins of mucus in the airways with SAα (2-3) gal moiety, which can provide MS a long respiratory colonization. The same type of desialylation for the NDV (strain La Sota) was shown.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 3456122Mycoplasma synoviae (MS) is a major poultry pathogen, most frequently causing subclinical respiratory tract infections which can progress to respiratory disease and infectious synovitis. MS is capable to invade host cells and also possess mechanisms to influence on the host’s immune system. MS binding and invading are mostly mediated by immunodominant surface lipoproteins VlhA and haemagglutinins. Among proteins that contribute to mycoplasma pathogenicity neuraminidase, cysteine protease and nucleases have been identified.
B.06 Other
COBISS.SI-ID: 2952328