This is the first study about communication at the microscale in B. subtilis and showed that remarkable diversity of the comQXPA QS system is present also at the microscale. The comQXPA is highly polymorphic in closely related strains and comprises four communication groups such that strains belonging to the same group exchange information efficiently, but strains from different groups not. In this work, using Bacillus isolates from the bank of the river Sava, we showed that the same number of communication types exists at the microscale (1 cm3) as was found among strains from macroscales.
COBISS.SI-ID: 3565432
We described new approach for the isolation and quantitative analysis of the cell wall proteins from invasively growing S. cerevisiae. This method is compatible with agar-invasion assays and is a screening tool for rapid analysis of proteins related to yeast adhesion and invasion. The extraction was optimized for fast, direct analysis of multiple protein samples by SDS-PAGE, avoiding preconcentration or purification. We compare protein profile of invasive and non-invasive strains, invasive and non-invasive part of the colony and cells cultivated at optimal and increased growth temperature.
COBISS.SI-ID: 3709816