The expression of bacterial polyketide synthase gene clusters is often controlled by a number of different families of regulatory proteins. We have undertaken a comparative bioinformatic analysis of the regulatory genes present in the oxytetracycline and chlortetracycline gene clusters of Streptomyces rimosus and S. aureofaciens. We have identified a new LAL(luxR)-family regulatory gene, otcG, in the otc gene cluster, that is not present in the ctc gene cluster. By gene disruption and over-expression experiments we have demonstrated a positive role of otcG in OTC biosynthesis.
COBISS.SI-ID: 3608696
In this article a robust and versatile reporter system was developed based on the rppA gene from Saccharopolyspora erythraea. The applicability of the reporter system has been demonstrated by expressing the rppA gene under the control of the heterologous promoters actII-ORF4/PactI, ermE and its upregulated variant ermE*. Studied reporter system has shown its utility independently of cultivation conditions or composition of growth medium, from simple laboratory to complex industrial media.
COBISS.SI-ID: 3811960
Yeast S. cerevisiae in the stationary phase was characterized at the cellular and proteome level. At the cellular level, optical density, cell viability, glycogen content, intracellular oxidation and cell energy metabolic activity were measured, while at the proteome level, protein profiles were analyzed using two-dimensional electrophoresis. The data obtained at both levels provide better insight into quiescence program state, which still remains poorly understood. At their base, optimal time period reflecting a stable metabolic and oxidative state of the yeast was determined. Consequently, this period is the appropriate to study changes in cell oxidant status and energy metabolic activity in response to different environmental stressors. Metodological approach at proteome level mentioned in the article was used also in our reserach project in the frame of WP4 and WP8.
COBISS.SI-ID: 3927928