The expression of bacterial polyketide synthase gene clusters is often controlled by a number of different families of regulatory proteins. We have undertaken a comparative bioinformatic analysis of the regulatory genes present in the oxytetracycline and chlortetracycline gene clusters of Streptomyces rimosus and S. aureofaciens. We have identified a new LAL(luxR)-family regulatory gene, otcG, in the otc gene cluster, that is not present in the ctc gene cluster. By gene disruption and over-expression experiments we have demonstrated a positive role of otcG in OTC biosynthesis.
COBISS.SI-ID: 3608696
Two different Verticillium albo-atrum pathotypes were analyzed by proteomic approach using 2-D electrophoresis for protein seperation, gel images analysis with software and identification of differentially expressed proteins by tandem mass spectrometry. Some of the methods, which were developed in the scope of this article, will be used during this research project.
COBISS.SI-ID: 5973881