In this article a robust and versatile reporter system was developed based on the rppA gene from Saccharopolyspora erythraea. The applicability of the reporter system has been demonstrated by expressing the rppA gene under the control of the heterologous promoters actII-ORF4/PactI, ermE and its upregulated variant ermE*. Studied reporter system has shown its utility independently of cultivation conditions or composition of growth medium, from simple laboratory to complex industrial media.
COBISS.SI-ID: 3811960
Proteins from the extracellular medium of Aeropyrum pernix K1 were separated by two-dimensional electrophoresis and identified using mass spectrometry. After identification bioinformatic analysis was done. Methodological approaches mentioned in the article are used also in our research project in the frame of WP4.
COBISS.SI-ID: 3750008