Yeast S. cerevisiae in the stationary phase was characterized at the cellular and proteome level. At the cellular level, optical density, cell viability, glycogen content, intracellular oxidation and cell energy metabolic activity were measured, while at the proteome level, protein profiles were analyzed using two-dimensional electrophoresis. The data obtained at both levels provide better insight into quiescence program state, which still remains poorly understood. At their base, optimal time period reflecting a stable metabolic and oxidative state of the yeast was determined. Consequently, this period is the appropriate to study changes in cell oxidant status and energy metabolic activity in response to different environmental stressors. Metodological approach at proteome level mentioned in the article is used also in our reserach project in the frame of WP4 and WP8.
COBISS.SI-ID: 3927928