Nosema and virus infections have supposedly increased colony mortality worldwide. We focused on the quality of the honeybee diet, winter survival and the influence of Nosema infections or viruses in colonies. Queens in the colonies were individually infected with spores of Nosema ceranae. N.apis/ceranae spores and viruses were diagnosed. The newly-emerged workers were individually inoculated with N. ceranae spores. Midguts were prepared for immunohistological analyses and the level of cell death was estimated.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 3401576Honeybee queens were examined in queen breeding stations across Slovenia during the rearing seasons 2006 and 2008. In both years, 612 queens from 27 apiaries were sampled. Queens were weighed, dissected and prepared for morphological measurements and pathogen analyses (Nosema spores and viruses). The attendants were also checked for spores, and Nosema spores were determined by PCR. In 2006, queens were ABPV, DWV and SBV positive. In 2008, DWV and BQCV were most frequently present in queens and attendants. Results of molecular tests found N. ceranae. Queens from 2006 were all virus negative.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 3401832