In this work we upgraded the kinetic studies of histamine uptake into neonatal rat cultured type 1 astrocytes with quantum chemical calculations of histamine pKa values in conjunction with Langevin dipoles solvation model as the first step toward microscopic simulation of transport. We demonstrated that histamine protonation states depend on the polarity of the environment as well as that histamine, a polar molecule at physiological pH uses at least two different mechanisms for its uptake into astrocytes –an electrodiffusion and Na+-dependent and ouabain sensitive active process.
Modelling of hydrological processes in karst aquifers is one of the biggest challenges in the field of hydrogeology. The paper presents conceptual approach of modelling that describes hydrological processes with combination of physically-based and empirical equations. This approach enables use of existing knowledge and data and simplification for those processes that cannot be accurately described. For the use in water management, modelling approach, which has been successfully tested in complex karst aquifer system, could be adapted to many other karst water resources.