We made a comprehensive analysis of tissue heating as a potential side effect of electric pulses used for electroporation-based treatments. The analysis is based on a coupled electrothermal model using 3-D finite-element approach. We used short, high-voltage, electropermeabilizing pulses and longer, lower voltage, electrophoretic pulses. The results show that at specific pulse parameters at least locally tissue heating may be significant. For electrochemotherapy, this is not critical, but DNA electrotransfer may be unsuccessful due to heating-related DNA damage or denaturation.
COBISS.SI-ID: 7351892
For an effective tissue electropermeabilization, it is very important to have information about the electric field distribution using a defined set of electrodes. Computer simulations based on finite element models showed field distribution to be very localized and highly homogeneous with the new concept of contact wire electrodes. They can be used to treat the skin and subcutaneous tumors. The electrodes after drug or DNA intradermal injection were validated by clinical treatment of large surface skin tumors and by in vivo imaging of permeabilization or of gene expression.
COBISS.SI-ID: 7006036