In this paper we describe a new method for the synthesis of magnetic liposomes with incorporated magnetic nanoparticles of iron oxide. The size of magnetic liposomes can be independently controlled by varying the intensity and frequency of electric field.
In this contribution we have shown that polymer surface is saturated with oxygen functional groups already in a very short time of plasma treatment. Among them especially important are carboxyl groups, which are needed for further binding of the protein streptavidin. Since functional groups are not stable with time, and since it is not always possible to immediately deposit the protein, we have studdied also aging of the functional groups during storage in water at different temperatures. We have found that low temperature can significantly reduce aging effects.
In this paper we have shown that by combination of plasma treatment and chemical coupling of protein streptavidin with the help of EDC reagent (1-ethyl-3-(3-dimethylaminopropyl) we can achieve very stable binding of protein to the surface of the substrate, while the physical adsorption itself was not so promising. Stable binding of protein to the plasma treated polymer surface can be achieved only with the chemisorption.