In this article, we report the identification of a previously unknown region of the FK506 gene cluster from S. tsukubaensis NRRL 18488 containing genes encoding provision of the unusual building blocks. We identified a group of genes, termed the “all” subcluster encoding biosynthesis of the extender unit allylmalonyl-CoA which provides the allyl group at C21 carbon atom of FK506. Interestingly, a small independent enzyme diketide synthase was identified to be involved in the biosynthesis of the allylmalonyl-CoA extender unit.
COBISS.SI-ID: 3754104
In this article was developed a robust and versatile reporter system based on the rppA gene from Saccharopolyspora erythraea. The applicability of the reporter system has been demonstrated by expressing the rppA gene under the control of the heterologous promoters actII-ORF4/PactI, ermE and its upregulated variant ermE*. The simplicity and robustness of the system, demonstrated even in industrial settings, shows great potential for wider use in different microbial hosts and applications, and may thus represent a new generic and versatile tool useful to a wider scientific community.
COBISS.SI-ID: 3811960