To improve clinical performance of licensed antibiotics and chemotherapeutic agents, it is important to understand the factors in Campylobacter that affect susceptibility to macrolide antibiotics. We demonstrated that EGCG increase Campylobacter spp. Susceptibilities and thus have effects on multidrug efflux systems that might improve the clinical performance of antibiotics. These findings provide an evaluation of a natural phenolic EGCG with modifying activities that can reverse macrolide resistance. Using mutants that lack the functional genes coding for the CmeB and CmeF efflux pump proteins and the CmeR transcriptional repressor, we show that these efflux pumps are potential targets for the development of therapeutic strategies that use a combination of a macrolide with an efflux pump inhibitor (EPI) to restore macrolide efficacy.
F.02 Acquisition of new scientific knowledgeCOBISS.SI-ID: 4119160
The article was published in a quality microbiological journal. We compared disk diffusion, agar dilution, broth microdilution and macrodilution methods. However, we established microdilution method with INT to indicate the viability of aerobic bacteria and with BacTitterGLO (ATP determination) as the only appropriate to indicate the viability for microaerophilic Campylobacter spp.. Gram negative pathogen Campylobacter spp. showed a similar sensitivity to plant extracts as the tested gram positive bacteria, but S. infantis and E. coli were more resistant. With this contribution we presented the fast screening method for MIC determination, which can give comparable results from different studies prior to their in vivo application. Using the method we determined the activity of several phenolic components and selected the most effective one for our future work.
F.02 Acquisition of new scientific knowledgeCOBISS.SI-ID: 3752312
The article was published in a quality microbiological and food science and tehcnology journal. We tested the antimicrobial effect of rosemary extracts against Campylobacter jejuni at a low storage temperature with or without shortterm prefreezing. Furthermore, low temperature storage conditions prolonged the survival of C. jejuni in chicken meat juice. The results in chicken meat food model again showed the synergistic effect of freezing and plant extract antimicrobial activity. A better understanding of how bacteria cope with stress conditions on one hand, and adapt to a protective environment on the other, will be critical in designing new intervention strategies for Campylobacter reduction in the poultry meat supply and control methods for food safety management.
F.02 Acquisition of new scientific knowledgeCOBISS.SI-ID: 3836280