The indirect importance of postodoctoral project is the cooperation, which were established during this time, with the prof. dr. Qijing Zhang (one of the most important on the filed of bacterial genetics, antibiotic resistance and pathogen-host interactions, Iowa State University, ZDA) and also with prof. dr. Franz Bucar (research interest in isolation of active plant constituents from medicinal plants of traditional medicines; resistance-modulation activity, Karl-Franzens-Univrsity, Graz, AU). The personal cooperation also increased in international collaboration by a bilateral project (SLO-ZDA, SLO-AU), by which our group will allow the achievement of the important goals. Through this joint project (in whic I cooperate as a researcher) several young researchers get the possibility to work in the important research laboratory and get the experience in genetic and chemical experimental work, what will significantly move the field forward also in our research institute and country. Within the collaboration several publications will be presented and also some under-graduation and post-graduation experimental work in which I cooperate as workin mentod or co-mentor, was based and will continue in the future.
F.02 Acquisition of new scientific knowledge
This article was published during the freezing of the project due to maternity leave. Since it is a result of the project and was not included in the first report (which I submitted in May 2010) I described in this report. The article was published in a quality microbiological journal. We compared disk diffusion, agar dilution, broth microdilution and macrodilution methods. However, we established microdilution method with INT (2-p-iodophenyl-3-pnitrophenyl-5-phenyl tetrazolium chloride) to indicate the viability of aerobic bacteria and with BacTitter-GLO (ATP determination) as the only appropriate to indicate the viability for microaerophilic Campylobacter spp.. Gram-negative pathogen Campylobacter spp. showed a similar sensitivity to plant extracts as the tested gram-positive bacteria, but S. Infantis and E. coli were more resistant. With this contribution we presented the fast screening method for MIC determination, which can give comparable results from different studies prior to their in vivo application. Using the method we determined the activity of several phenolic components and selected the most effective one for our future work.
F.01 Acquisition of new practical knowledge, information and skillsCOBISS.SI-ID: 3752312